Crohn's Disease Forum » Books, Multimedia, Research & News » Lactobacillus casei Prevents NSAID-Induced Small Intestinal Damage


02-04-2017, 05:49 PM   #1
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Lactobacillus casei Prevents NSAID-Induced Small Intestinal Damage

See new study: https://www.karger.com/Article/FullText/452356

Abstract

Inflammatory responses triggered by activation of the lipopolysaccharide (LPS)/Toll-like receptor (TLR) 4 signaling pathway are a key mechanism in nonsteroidal anti-inflammatory drug-induced enteropathy. The aim of this study was to investigate the probiotic effect of Lactobacillus casei strain Shirota (LcS) on indomethacin-induced small intestinal injury. Rats pretreated with viable LcS or heat-killed LcS once or once daily for a week were administered indomethacin by gavage to induce injury. Anti-inflammatory effects of l-lactic acid (115 mM) were evaluated in vitro by use of THP-1 cells. One-week treatment with viable LcS prevented indomethacin-induced intestinal injury with increase in the concentration of lactic acid in small intestinal content and inhibited increases in myeloperoxidase activity and expression of mRNA for tumor necrosis factor-α (TNF-α) while affecting neither TLR4 expression nor the number of gram-negative bacteria in intestinal content, whereas neither heat-killed LcS nor a single dose of viable LcS inhibited intestinal injury. Prevention of this injury was also observed in rats given l-lactic acid in drinking water. Both l-lactic acid and LcS culture supernatant containing 10 mM lactic acid inhibited NF-κB activation and increases in TNF-α mRNA expression and TNF-α protein secretion in THP-1 cells treated with LPS. Western blot analyses showed that both l-lactic acid and LcS culture supernatants suppressed phosphorylation and degradation of I-κB-α induced by LPS without affecting expression of TLR4. These findings suggest that LcS exhibits a prophylactic effect on indomethacin-induced enteropathy by suppressing the LPS/TLR4 signaling pathway and that this probiotic effect of LcS may be mediated by l-lactic acid.
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